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Eugeal was the first to go on a mission. The other four were shadowed. Eugeal drove a car and used a gun to extract her targets' Pure Hearts herself, and send a Daimon Heart Snatcher in a box to deal with the Sailor Scouts whenever they tried to stop her. She usually attacked with a flamethrower. She nearly got the Purity Chalice once it awakened, but Sailor Moon got it first and used it to defeat her. She drove away in her car, but as Mimet put acid snails in her car to dissolve the brakes, Eugeal crashed over the edge of a cliff.
Mimet was the next to go on a mission. She would always target well-known young men, and send Daimons kept in a briefcase to suck out the targets' Pure Hearts through their mouths. She attacked by shooting a star beam from her staff that would disorient the victim. After seven failures, Doctor Tomoe decided to give the next job to Telulu, but Mimet tried to trick Telulu and took the job anyway. Telulu figured her out, though. After Mimet's monster was destroyed, she entered Eugeal's enlarging machine to increase her size and power, but Telulu got her revenge by pulling the plug on her, trapping her inside it forever, and she fell into nothingness. Each of the remaining witches only lasted a single episode against the Sailor Scouts.
Telulu was the third to go on a mission. She opened a plant shop and created plants called Venus Heart Traps that would steal a person's Pure Heart. She attacked by extending vines from her hands and electrocuting her victims with them. Once Sailor Moon destroyed all but one of her plants, she injected the last plant to turn it into Big Bertha, a plant monster. However, Tuxedo Mask shattered Telulu's containment device for Pure Hearts, scattering them around her and subsequently turning Big Bertha against her in pursuit of the hearts. When Telulu electrocuted Big Bertha, she selfdestructed, killing them both.
Byruit was the fourth to go on a mission. At the academy that Sailor Mercury attended, she used her master computer, Nano, to suck the exam takers' Pure Hearts out through their computers. She was also armed with a nanocuff that shot a ray of nanomachines (Mosaic Energy Blaster) to slowly dissolve her opponents, only leaving their Pure Hearts (if they had one). However, Sailor Moon damaged her nanocuff, causing the nano ray to backfire on her and dissolve her instead, destroying Nano and releasing the Pure Hearts.
Cyprin, the strongest of the witches, was the fifth to go on a mission. She shares her mind and body with Petirol, and they attack with energy beams from their staffs. They used a statue of Mistress 9 to extract the Pure Hearts of the students at Mugen School. The Sailor Scouts fought them, and though they proved to be extremely formidable, Sailor Mars and Sailor Jupiter tricked them into blasting each other, destroying them both.