American McGee's Alice. They work for the Mad Hatter, running his asylum in Looking Glass Land, and they are none-too-kind to guests
When Alice encounters them, she is reminded of the orderlies at Rutledge Asylum whose sadistic teasing and lascivious taunts are analogous to the Tweedles. These orderlies were revealed to also be twins, and nephews of the Rutledge superintendent, who often forcefed Alice until she got revenge by stabbing one in the cheek with a spoon.
Tweedledee and Tweedledum are dimwitted lunatics capable of melee attacks, using their propeller beanies to fly and drop down on Alice, and the ability to split open and spawn smaller, weaker versions of themselves. According to the official collectible figurines, the larger Tweedle is Tweedledee, and the smaller Tweedle is Tweedledum.