Spectral Ghostbusters (also known as the Anti-Ghostbusters) appeared in the animated series The Real Ghostbusters in episode, "Citizen Ghost" and are the results of the battle against Gozer which has manifested into evil doppelgängers using the Ghostbusters' old uniforms.

In a way, Peter Venkman was responsible for their creation (as they were created from the duties he was too lazy to perform). The mission of the Spectral Ghostbusters was to get rid of the Ghostbusters.