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Sofie Fatale is present at the "House of Blue Leaves" when the Bride arrives to kill O-Ren Ishii. The Bride is in the bathroom when Sofie walks in and answers her phone; the Auld Lang Syne ringtone is recognized by the Bride. She remembers that Sofie was present during the El Paso massacre, but did not assist in beating the Bride. The Bride captures Sofie and brings her to O-Ren Ishii. The Bride then cuts off Sofie's left arm (the arm she holds her phone with) as a means of both enacting revenge on her and showing Ishii her intentions for a duel.
Later, the Bride interrogates Sofie to learn the location of the rest of the members of the Deadly Viper Assassination Squad, cutting off her right forearm and threatening to further dismember her if she refuses to speak. After cutting her other arm she tells the Bride everything she wants to know. She then sends her rolling down a hill to a hospital for medical attention so she can live on and tell Bill what has happened and that the Bride is still alive. Bill visited her in the hospital where Sofie relayed that to him.
- The Japanese-released version included a scene where The Bride cut off Sofie's other arm, while the Western-released version shows her with that arm.
- Tarantino has revealed plans for Vol. 3 where Sofie inherits Bill's and O-Ren's money and raises Vernita Green's daughter Nikki to get revenge.