He is portrayed by Dougray Scott who also played Niles York in the 2013 film Death Race 3: Inferno and Stuart John in the 2015 film Taken 3.
Sean Ambrose was a man who determined himself to become rich by selling the Chimera virus. Though he thought he is caring, he is quick to aggression and showed himself to be possessive and arrogant. Examples are when he refused to listen to Hugh's warning of Nyah's return or pointing his gun at McCloy when he questioned him while torturing Hugh (disguised as Hunt). Also as a former IMF Agent, he is very perceptive to IMF's tactics as noted when he grabs Hugh who implies that Nyah's sudden return was alluding to Hugh himself of possibly a mole in his group sent by IMF to spy on them. Particularly, his former colleague Agent Ethan Hunt since he was previously ordered to double as him during missions when Hunt was needed but unavailable to answer. In fact, it is possible that him doubling for Hunt on various missions developed a resentment towards his former employer and colleague in particular as it is possible that he almost never allowed to show his real face during most of his time in his spy career. Despite his flaws, Ambrose was extremely intelligent and calculating, planning a plane assault with his men, and gaining Nyah's information while disguising as Hunt. He was also suave and somewhat caring for his own allies, notably Nyah. When she returned, he showed himself to be happy and joyful with her at the horse race. Despite his care for her, he implied his usage of sex to Hugh before while using the cigar cutter. When he killed Hugh, he appeared to be horrified by his actions, showing he cared for him somewhat.
That being said, he could turn on others, showing ruthlessness if needed. Notably when Nyah injected herself with Chimera, he called her "bitch" in frustration, but did not kill her because she had £37,000,000 worth in her. When McCloy became disturbed by his violent act of torturing the masked Hugh, he pointed his gun at the latter. And when Hugh questioned him on Nyah's return, he use his cigar cutter on him.
Ambrose was also a man of enjoyment and riches, wearing expensive clothing and gambling at the horse racing event. He strongly enjoyed Cuban cigars.
- Sphinx AT 2000: Ambrose's signature sidearm throughout the film.
- Heckler & Koch USP Compact: Ambrose's secondary sidearm as shown when it was dropped on the beach where Hunt and Ambrose had a final showdown. As Ambrose attempts to shoot Hunt with his own Beretta 92FS, the latter finds Ambrose's own backup sidearm and uses it to shoot Ambrose three times and kill him for good.
- Beretta 92FS: Hunt's signature sidearm for the film, Ambrose uses a 92FS in an attempt to shoot Hunt before his own death.
Ambrose's terrorist group
- Hugh Stamp (right-hand man)
- Several other operatives
Ambrose's business partner
- John Chaddick McCloy (Chief Executive Officer of Biocyte Pharmaceuticals)
|“||Hunt's target will be the Chimera, stored and manufactured at Biocyte on the 42nd floor.||„|
|~ Sean Ambrose briefing his men about Hunt's mission|
|“||Shh. Listen to me. There's no time to talk. It is critical, absolutely critical that you do whatever Ambrose asks. Do you understand me?||„|
|~ Sean Ambrose, disguising as Ethan Hunt, talks to his lover Nyah|
|Mission: Impossible Villains|