Sean Ambrose is a former IMF agent who is the main antagonist of the 2000 film Mission: Impossible II. He is portrayed by Dougray Scott.
Ambrose's past is unknown, but he was presumbly born in the United Kingdom as stated by his nationality and he joined as an agent for an independent espionage agency commonly works for the United States government, Impossible Mission Force, before he attempts to get his hands on a new deadly virus, Chimera, as well as its cure, Bellerophon. Ambrose has sold the virus for £37,000,000, and he intends to release the virus into the civillian population and then sell the cure to the highest bidder.
Ambrose uses an Ethan Hunt latex mask to board Dr. Vladimir Nekhorvich's plane and steals Chimera samples from the doctor who had infected himself with a sample after killing him and escapes with three of his men; Wallis, Ulrich and Hugh, as the plane crashes in the Rockies. Meanwhile, Ethan is assigned a mission of recovering the virus with the help of three members of his team (one of them being a professional thief Nyah Nordoff-Hall), and he manages to destroy a number of Chimera samples inside the head office of Biocyte Pharmaceuticals. Before Ethan can destroy the final sample, Ambrose and his men appear and engage Ethan in a firefight. He forces Nyah to get the virus for him, but she injects herself with it before he can stop her. Ambrose kidnaps Nyah and leaves, knowing that she is now valuable to him, but Ethan knows that if he does not cure Nyah with Bellerophon within twenty hours remaining and left unchecked, she will die along with 20,000,000 people in Australia.
Later, Ethan's team track Ambrose's men to Biocyte storage bunker during a meeting and Ethan successfully steals Bellerophon and Chimera samples from them. Meanwhile, Ethan's team in the helicopter locate Nyah on top of the northern head bluff, who is about to jump from the cliffs and kill herself to prevent the virus inside her from spreading. Ethan and Ambrose have another firefight during a frantic motorcycle chase until the pair eventually fall onto a beach before engaging in a fistfight, with Ethan gaining the upper hand and knock Ambrose out. However, the latter recovers and laughs triumphantly as he points Ethan's Beretta 92FS at his back. Ethan notices Ambrose's USP Compact half buried in the sand by his feet and kicks it into the air, grabs and spins around after tossing Bellerophon to his good friend, Luther Stickwell, for him to cure Nyah on time. As Ambrose shoots at him, Ethan drops to the ground and shoot Ambrose three times in the chest and back, killing him for good.
Ambrose's known associates
- Hugh Stamp (right-hand man)
- Several other operatives
- John C. McCloy (business partner)