He is portrayed by Randy Couture.
Sarkhan trains young warriors to become Black Scorpions who are the elite bodyguards of Akkadian royality, and Mathayus takes part in his training course to become a Black Scorpion himself to avenge his father, who was murdered by Sargon. When Mathayus finishes his training, he learns that Sargon has become the new king of Akkad, having presumably murdered the previous king and taken his place. Noticing Mathayus as a competent warrior and swordsman, Sargon tells him to come to his palace to work for him. When Mathayus arrives, Sargon asks him to prove his loyalty to him by killing his brother, Noah, who had been heard blaspheming Sargon. Mathayus saves Noah by killing Sargon's guards and hurls a spear at the king, who repels the weapon with magic. Mathayus escapes from the city with Noah, but Sargon fires a magic arrow which follows them and kills Noah. Mathayus then travels to Egypt in hopes of retrieving a special sword known as the Sword of Damocles, which is said to be able to kill Sargon.
Later, Mathayus ventures into the underworld and takes the Sword of Damocles from the goddess Astarte. Knowing that Mathayus will try and use it to Jill Sargon, Astarte appears before Sargon and asks him to retrieve the sword for her. Sargon claims he will need more power to accomplish this, and she grants his request. Mathayus and his followers return to Akkad where Sargon is planning to torch all of his citizens, and Mathayus is then approached by his father. This turns out to be Sargon in disguise, and after engaging in hand-to-hand combat, Sargon transforms into a giant invisible scorpion. Mathayus manages to make Sargon visible by throwing black oil over him, and after avoiding a barrage of attacks, Mathayus stabs him with the Sword of Damocles. Sargon screeches in pain and collapses dead on the palace steps.