He was portrayed by the late Gian Maria Volontè, who also played El Indio in the sequel For a Few Dollars More.
He is first seen when Blondie rides to where the Mexican army is doing a trade with the Americans, but the army is massacred when the Americans turn out to be Ramón and the Rojos in disguise. Afterwords, Blondie tricks the Rojos and the Baxters to go to the cemetery to find two "survivors" of the massacre, really two dead Mexican soldiers leaning up against a grave.
Ramón is able to "kill" the "survivors" and capture Antonio Baxter in the process. While the fighting happens, Blondie searches for the gold that they stole, but hides when he hears someone coming. It turns out to be Marisol, Ramón's mistress. Unfortunately, Blondie doesn't realize this until after he knocks her out. He takes her to the Baxters to help her, and they use her to trade for Antonio. Blondie learns of her past, and frees her, causing all hell to break loose while Ramón searches for both of them.
Ramón captures Blondie and brutally interrogates him, but Blondie escapes. Ramón thinks that the Baxters are protecting him, so he sets fire to there house and kills all the residents. The next day, Ramón is now interrogating Silvanito, but Blondie interrupts them. After a standoff, Blondie kills all of Ramón's guards, including Rubio, and challenges Ramón to see if he can reload his rifle faster than he can reload his revolver. He fails and Blondie shoots him in the chest, killing him.
Ramón is described as the most capable and intelligent of the Rojos Brothers. He is extremely cruel, vicious, ruthless and sadistic, does not hesitate to kill, can kill an entire army of men seen at the beginning of the film. He also feels a remarkable racism toward Americans. He prefers a Winchester Rifle and views rifles as superior to pistols.