The Parent Teacher Organization of Eradicating Youngsters is a small group of five adult villains including both parents and teachers, as their name implies. They are a parody of the PTA. Like many villains, the members of the group represent archtypes of oppressive authority figures, in this case those related to school.

They consist of The Principal, a school principal and the de facto leader of the group, who can fly but seems to do little else, Mad Dad, an abusive father figure who is angry with his child's poor grades, voiced by Jeff Bennett, The Terrible Tutor, a a school tutor who can shoot textbooks from his sleeves, voiced by Dee Bradley Baker, Heli-Teacher, an old woman possessing a flying chair, voiced by Cree Summer, and Midwestern Mom, a wrestler-like mother figure, voiced by Grey DeLise.

Their only major appearance was in Operation: N.O.-P.O.W.U.H., in which they attack the Sector V Treehouse but are quickly defeated. Aside from this, the members of the group are frequently seen at large gatherings of villains.