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Mike Tyson appears as a boss and minor villain in the comedy video-game "I Wanna Be The Guy" and is a heavily modified version of the non-villainous boss from Mike Tyson's Punch Out (after all Tyson is a boxer, thus his appearance in Punch Out was not antagonistic as he was simply defending his title).
In "I Wanna Be The Guy" however Tyson is definitely out to kill and is no longer a mere boxer but a literal giant - though his attacks consist mainly of powerful punches just like he did in Punch Out, only magnified greatly due to his size.