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100-mario-donkey-kong-jr
While Mario is depicted as a hero through most of his history, he is enemies with Donkey Kong and Donkey Kong Jr.. These are his villainous actions. Coincidentally, Mario in his first game, the original Donkey Kong, was depicted as a naughty carpenter who abused his own pet, Donkey Kong. Also in the prequel Game & Watch game, Donkey Kong Circus, Mario forces poor Donkey Kong to perform at the circus by juggling pineapples and dodging fireball, and even laughs at him whenever he either gets hit by a fireball or drops a pineapple. His role just worsened in the sequel, Donkey Kong Jr. as he is actually the villain in that game.

Donkey Kong Jr. (game)

Wpid-donkey-kong-jr.-screenshot

Mario went on to imprison Donkey Kong in a cage. DK Junior went on to try and rescue his father. Mario attempted to stop the young ape and released various types of traps mainly consisting of animals. Junior eventually did make it to his father and the two returned to Donkey Kong Island just after Donkey Kong made a shot at Stanley the Bugman's greenhouse.

He also whipped the animals that worked for him, eventually known as Mario's slave labor, making him extremely evil, as he also let unnecessarily made them know who was in charge (him) his role wasn't better before as he abused DK before the events of Donkey Kong. Jr.

           Mario Villains

Koopa Troop
Bowser | Bowser Jr. | Koopalings: (Larry Koopa | Morton Koopa Jr. | Wendy O. Koopa | Iggy Koopa | Roy Koopa | Lemmy Koopa | Ludwig Von Koopa) | King Boo (Luigi's Mansion) | Boos

8 Bit Club
Wart | Mouser | Tryclyde | Fryguy | Clawgrip | Birdo | Shy Guys

Secret Society of X-Nauts
Sir Grodus | Lord Crump | Shadow Sirens | Shadow Queen

Count Bleck's Minions
Count Bleck | Nastasia | O'Chunks | Mimi

Smithy Gang
Smithy | Exor | Mack | Bowyer | Yaridovich | Axem Rangers | Blade | Boomer | Count Down | Cloaker | Domino | Clerk | Manager | Director | Factory Chief

Kremlings
King K. Rool | Kremling Krew | Klump | Krusha | Krunch | Klubba

Other
Antasma | Ashley | Bonechill | Brobot | Cackletta | Chuckolator | Cortez | Dark Star | Dimentio | Dino Piranha | Donkey Kong | Doopliss | Draggadon | Eely-Mouth | Elder Princess Shroob | Fawful | Gooper Blooper | Grubba | Kent C. Koopa | King Bob-omb | Necky | Army Dillo | Wizpig | Kaptain Skurvy | King Kaliente | Mr. L | Nabbit | Petey Piranha | Peewee Piranha | Rabbids | Shroobs | Tatanga | Sunnycide | Squizzard | Viruses | Waluigi | Wario | Wracktail | Wingo | Mad Jack