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100-mario-donkey-kong-jr
While Mario is depicted as a hero through most of his history, he is enemies with Donkey Kong and Donkey Kong Jr.. These are his villainous actions.

Coincidentally, Mario in his first game, the original Donkey Kong, was depicted as a naughty carpenter who abused his own pet, Donkey Kong.

In the prequel Game & Watch game, Donkey Kong Circus, Mario forces poor Donkey Kong to perform at the circus by juggling pineapples and dodging fireball, and even laughs at him whenever he either gets hit by a fireball or drops a pineapple.

His role just worsened in the sequel, Donkey Kong Jr. as he is actually the villain in that game.

Donkey Kong Jr. (game)

Wpid-donkey-kong-jr.-screenshot

Mario went on to imprison Donkey Kong in a cage. DK Junior went on to try and rescue his father. Mario attempted to stop the young ape and released various types of traps mainly consisting of animals. Junior eventually did make it to his father and the two returned to Donkey Kong Island just after Donkey Kong made a shot at Stanley the Bugman's greenhouse.

He also whipped the animals that worked for him, eventually known as Mario's slave labor, making him extremely evil, as he also let unnecessarily made them know who was in charge (him) his role wasn't better before as he abused DK before the events of Donkey Kong. Jr.

Donkey Kong Circus

Mario circus

Mario appears as the antagonist in Donkey Kong Circus. Mario is an audience member who laughs at Donkey Kong, the new attraction of the circus, whenever he drops a pineapple or gets hit by fire. In the end of the game, Mario is shown in the construction site where the first game begins.

           Donkey Kong Villains

Kremlings
King K. Rool | Kaptain Skurvy | Kritter | Klump | Kip | Kass | Kalypso | Kludge | Krusha | Kudgel | Congazuma | Kackle | Klaptrap | Klobber | Koin | Kosha | Kerozene | Kopter | Kuff 'n' Klout | Kutlass | Mega Amp | Dragon Kremling | Kloak
Allies
KAOS | Very Gnawty and Really Gnawty | Master Necky and Master Necky Snr. | Queen B. | Dumb Drum | Flotsam | Clambo | Krow | Kleever | Zinger | Gnawty | Army | King Zing | Manky Kong | Slippa | Belcha | Arich | Squirt | Bleak | Barbos
Tiki Tak Tribe
Tiki Tong | Kalimba Tiki | Gong Tiki | Maraca Tikis | Panflute Tiki | Accordion Tiki | Banjo Tiki | Xylophone Tiki | Tiki Tong Tower | Tiki Goon | Screamming Pillar | Tiki Buzz | Tiki Pop | Tiki Bomber | Tiki Tank | Tiki Seeker | Tiki Torch | Tiki Boing
Allies
Mugly | Scurvy Crew | Stu | Mole Train | Mangoruby | Thugly | Colonel Pluck | Awk | Toothberry | Squeeklys | Snaps | Chomps | Snaggles | Squiddicus
Snowmads
Lord Fredrik | Pompy | Skowl | Ba-Boom | Fugu | Bashmaster | Tuck | Waldough | Hootz | Fluff | Lemmington
Allies
Dozy | Buffaloafer | Schnautzel | Swooper Dooper | Ice Dragons
Others
Fire | Snapjaw | Mario | Wizpig | Krunch | Crazy Kong | Fire Necky | Sassy Squatch | Davy Bones | B. Locker | Krosshair | Kroctopus | Kong Fu | Cactus King | Hogs | Tusks | Rocs | Dread Kong | Sumo Kong | Karate Kong | Ninjape | Iguanagon | Smokey the Dragon | Bluey the Walrus | Bubbler the Octopus | Tricky the Triceratops | Army Dillo | Dogadon | Mad Jack | Puftoss | King Kut Out | Hard Hat | Screech | Polly Roger | Chomps