Madame Boss is a villain from the Pokémon anime series. She is the founder and former leader of the notorious organization Team Rocket, bent on capturing and exploiting Pokémon to take over the world. She is also revealed to be the mother of Giovanni (the major antagonist of the entire Pokémon series), and only appeared in the radio drama The Birth of Mewtwo.
Nothing much is known about Madame Boss, other than her desire to capture and sell Pokémon for money and power. Despite her power as the leader of Team Rocket, the only Pokémon she desired to have was the legendary Mew. She manages to send her favored agent Miyamoto (the mother of future Team Rocket agent Jessie) to capture Mew, although Miyamoto ends up disappearing in a blizzard after her team finds a fossilized Mew eyelash. With the DNA recovered from the eyelash, Madame Boss gets her scientists to create a clone of Mew called Mewtwo.
However, Madame Boss had passed away by unknown causes during the creation of Mewtwo. Before her death, Madame Boss passed down her position to her son Giovanni, who takes over her remaining operations as the new leader of Team Rocket. Taking on his mother's legacy, Giovanni uses Mewtwo to capture hundreds of Pokémon for the organization, although this all ended up in failure when Mewtwo rebelled against Giovanni after learning that he was using him for his plans of conquest.