The Lonesome Ghosts are the main antagonists in the Disney animated short of the same name and three of the tertiary antagonists in Mickey's House of Villains. They like to play tricks on anybody who enters their house.

The Lonesome Ghosts first got bored and tricked Mickey, Donald, and Goofy into calling them to their house. When the trio entered the ghost's house, the Lonesome Ghosts started to play tricks on them. Mickey, Donald and Goofy then fell into some flour and molasses, giving them a disguise as creepy ghosts. Then they scared the Lonesome Ghosts away from their house and ran off into the distance. Donald then calls out, "So ya can't take it, ya big sissies?" Amen to that.