Jean Bison first appeared in Episode 2: "A Starry-Eyed Encounter", along with the first appearances of the Contessa and Arpeggio, at a ball Rajan was hosting. It is safe to assume he was a VIP at the event. He later appeared beside Rajan as they watch Sly dance with Neyla, commenting that he wished Rajan's spice shipments moved as well as Sly.
Despite his backwards, low-tech ways and old-style thinking, Jean Bison ended up with four of the Clockwerk parts: the Lungs, Stomach< and Talons. He attached the Lungs and Stomach to his three Iron Horse trains, allowing them to run day and night without stopping. However, Sly and the gang were able to pull off some good old-fashioned train robberies, stealing all three parts despite his precautions. Before they did so, Sly overheard talk between Jean Bison and Arpeggio, who seemed to be his close partner, on a speaker phone about their plans with the Clockwerk parts.
After losing three of his Clockwerk parts, Jean Bison escaped to his large lumber camp on the coast of Northern Canada and continued his true operation—using his giant attractor to collect the light energy of the Aurora Borealis into his Northern Light Battery for Arpeggio's blimp. He also began preparations for his Lumberjack Games, a competition for which he put up his Clockwerk Talons - that he'd been using to cut down trees - as a trophy. This drew Sly and the Cooper Gang like moths to a flame. After setting up the northern lights battery for their transport to Arpeggio's blimp, they entered the games.
Despite stellar performances and cheating to make Jean Bison lose, the three were captured. While the Cooper Gang was in his custody, Bison had his men raid the gang's hideout and steal all of the Clockwerk Parts that the gang had collected and sold them to Arpeggio (including the talons). Bentley was then forced into a battle with Bison and, using the lumber camp's equipment, Bentley was able to beat him.
According to the ending of Sly 2, although he was not arrested, Jean Bison somehow turned over a new leaf and went to work for the EPA, and froze yet again on a mission to save baby penguins.