Jay, Raven and Lark are the bodyguards and henchwomen of the Penguin and enemies of Batman. They are also waitress at the Penguin's club, "The Iceberg Lounge" and help Penguin with his underworld businesses. Their boss also sent them to do other dirty work like eliminating anyone that stands in his way or knows too much about his secret dealings. Killer Croc and Two-Face are also friends of Jay, Raven and Lark. They protect the Penguin's secret dealings from the public to ensure that he has legitimate appearance.
The trio were first sent by Penguin to eliminate Roxy Rocket, who had formerly employed by the Penguin, but were taken down by Roxy and Batman.