They were hired to help Mr. Freeze to make the citizens of Gotham miserable and destroy their dreams. The group first destroyed an ancient dinosaur skeleton and destroyed a scientist's dreams. Mr. Freeze and his goons interrupted a celebration when he freezes a prized painting into destruction. Batman arrives and fights Freeze and his goons. One of the maidens points a freeze gun at Batman but deflects the pan, freezing her leg. Mr. Freeze and two of his ice maidens retreated, leaving one of the ice maidens behind.
Freeze targeted Wayne Manor and seeks to make Bruce Wayne miserable. They break into the Manor and slowly freeze Alfred. Batgirl arrives and knocks the freeze gun out of Mr. Freeze’s hand while Bruce and Tim overpower the Ice Maidens. Freeze breaks out of the ice and an ice maiden knocks out Batgirl and Tim. They drove back to the laboratory and kept the doctors as hostages to find out their boss’s condition. Batman and Batgirl infiltrated the lab but were caught and Mr. Freeze revealing his condition. Freeze allows them to live so they could see Gotham frozen. After Mr. Freeze leaves, Batgirl disarms the Ice Maidens and holds them off and takes them to custody.