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Donkey Kong is shown as a hero in the Donkey Kong Country series, and a recurring character in the Mario spin-off games. But in the Mario vs. Donkey Kong series, Donkey Kong becomes Mario's rival, similar to his predecessor who is currently Cranky Kong.

Mario vs. Donkey Kong

In the first game, Mario vs. Donkey Kong, when Donkey Kong heard about the new Mini-Mario toys, he rushed to the store to get his very own, but then he saw that they were already sold out. Then Donkey Kong came across a factory making Mini-Marios.  So he breaks in, and takes some Mini-Marios from the working Toads.  Then Mario catches him and spends the rest of the game trying to stop him from stealing the Mini Marios, as well as kidnapping the working Toads.  After several journeys and battles of Mario rescuing the Mini Marios and Toads, Donkey Kong was sobbing while defeated. Mario then decided to console him and offer him one Mini Mario toy, which Donkey Kong happily accepted.

In other Mario vs. Donkey Kong games

In the second game, March of the Minis, during the grand opening and ribbon-cutting ceremony of a new amusement park, "Super Mini-Mario World", Mario offers Pauline, his VIP guest, a Mini-Mario.  Donkey Kong, wanting to win Pauline's heart, offers her a Mini-Donkey Kong, but Pauline chose the Mini-Mario instead. Enraged, Donkey Kong ran through with Pauline, and Mario uses the Mini-Marios to save Pauline from Donkey Kong.

In Minis March Again, Mario and Pauline were selling Mini-Marios, and Donkey Kong was last in a long line. Then when Donkey Kong was about to get one, they ran out, so Donkey Kong kidnaps Pauline again, and Mario gets the Mini-Marios to track down Donkey Kong and rescue Pauline again.

In Mini-Land Mayhem, Mario and Pauline open another amusement park, "Mini-Land", and offers the first 100 guests a free Mini-Pauline. Donkey rushed for one, but Mario pointed out that he was the 101st guest and he can't get one, so Donkey Kong gets mad, and runs through the park, capturing Pauline again, so Mario gets the Mini-Marios to rescue Pauline from Donkey Kong once again.

           Mario Villains

Koopa Troop
Bowser | Bowser Jr. | Koopalings: (Larry Koopa | Morton Koopa Jr. | Wendy O. Koopa | Iggy Koopa | Roy Koopa | Lemmy Koopa | Ludwig Von Koopa) | King Boo (Luigi's Mansion) | Boos

8 Bit Club
Wart | Mouser | Tryclyde | Fryguy | Clawgrip | Birdo | Shy Guys

Secret Society of X-Nauts
Sir Grodus | Lord Crump | Shadow Sirens | Shadow Queen

Count Bleck's Minions
Count Bleck | Nastasia | O'Chunks | Mimi

Smithy Gang
Smithy | Exor | Mack | Bowyer | Yaridovich | Axem Rangers | Blade | Boomer | Count Down | Cloaker | Domino | Clerk | Manager | Director | Factory Chief

Kremlings
King K. Rool | Kremling Krew | Klump | Krusha | Krunch | Klubba

Other
Antasma | Army Dillo | Ashley | Bonechill | Brobot | Cackletta | Chuckolator | Cortez | Dark Star | Dimentio | Dino Piranha | Donkey Kong | Doopliss | Draggadon | Eely-Mouth | Elder Princess Shroob | Fawful | Gooper Blooper | Grubba | Kent C. Koopa | King Bob-omb | Necky | Wizpig | Kaptain Skurvy | MegaBug | King Kaliente | Mr. L | Nabbit | Petey Piranha | Peewee Piranha | Phantom of the Bwahpera | Rabbids | Rabbid Kong | Shroobs | Tatanga | Sunnycide | Squizzard | Viruses | Waluigi | Wario | Wracktail | Wingo |

           Donkey Kong Villains

Kremlings
King K. Rool | Kaptain Skurvy | Kritter | Klump | Kip | Kass | Kalypso | Kludge | Krusha | Kudgel | Congazuma | Kackle | Klaptrap | Klobber | Koin | Kosha | Kerozene | Kopter | Kuff 'n' Klout | Kutlass | Mega Amp | Dragon Kremling | Kloak
Allies
KAOS | Very Gnawty and Really Gnawty | Master Necky and Master Necky Snr. | Queen B. | Dumb Drum | Flotsam | Clambo | Krow | Kleever | Zinger | Gnawty | Army | King Zing | Manky Kong | Slippa | Belcha | Arich | Squirt | Bleak | Barbos
Tiki Tak Tribe
Tiki Tong | Kalimba Tiki | Gong Tiki | Maraca Tikis | Panflute Tiki | Accordion Tiki | Banjo Tiki | Xylophone Tiki | Tiki Tong Tower | Tiki Goon | Screamming Pillar | Tiki Buzz | Tiki Pop | Tiki Bomber | Tiki Tank | Tiki Seeker | Tiki Torch | Tiki Boing
Allies
Mugly | Scurvy Crew | Stu | Mole Train | Mangoruby | Thugly | Colonel Pluck | Awk | Toothberry | Squeeklys | Snaps | Chomps | Snaggles | Squiddicus
Snowmads
Lord Fredrik | Pompy | Skowl | Ba-Boom | Fugu | Bashmaster | Tuck | Waldough | Hootz | Fluff | Lemmington
Allies
Dozy | Buffaloafer | Schnautzel | Swooper Dooper | Ice Dragons
Others
Fire | Snapjaw | Mario | Wizpig | Krunch | Crazy Kong | Fire Necky | Sassy Squatch | Davy Bones | B. Locker | Krosshair | Kroctopus | Kong Fu | Cactus King | Hogs | Tusks | Rocs | Dread Kong | Sumo Kong | Karate Kong | Ninjape | Iguanagon | Smokey the Dragon | Bluey the Walrus | Bubbler the Octopus | Tricky the Triceratops | Army Dillo | Dogadon | Mad Jack | Puftoss | King Kut Out | Hard Hat | Screech | Polly Roger | Chomps

           Super Smash Bros. Villains

Subspace Army
Tabuu | Master Hand | Primids | Ganondorf | Bowser | Ancient Minister | Wario | False Peach | False Zelda | Petey Piranha | Rayquaza | Ridley | Galleom | Duon | Porky Minch/King Statue

Playable Characters
Bowser | Bowser Jr. | Dark Pit | Donkey Kong | Ganondorf/Ganon | Koopalings | Mewtwo | Ryu | Wario | Wolf O'Donnell

Bosses
Giga Bowser | Crazy Hand | Dark Emperor | Master Hand | Master Core | Mumkhar | Petey Piranha | Porky Minch | Ridley | Tabuu | Yellow Devil |

           DEATH BATTLE Villains

DEATH BATTLE Combatants
Boba Fett | Shang Tsung | Akuma | Zangief | Goomba | Koopa | Riptor | Kratos | Shadow the Hedgehog | Vegeta | Starscream | Dr. Eggman | Eggman Empire | Badniks | Silver Sonic | Mecha Sonic | Mecha Knuckles | E-101 Beta | Shadow Androids | Scratch | Grounder | Metal Sonic | Dr. Wily | Yellow Devil | Guts Man | Metal Man | Slash Man | Magnet Man | Sheep Man | Napalm Man | Pharaoh Man | Bass | Treble | M. Bison | Shao Kahn | Ivy Valentine | The Terminator | Sektor | Fulgore | Gamera | Godzilla | Ryu | Scorpion | Deathstroke | Deadpool | Majin Buu/Kid Buu | Gaara | Segata Sanshiro | Nightmare/Inferno/Soul Edge | Lex Luthor | Black Beast | Darth Vader | Doctor Doom | Donkey Kong | Trish | Jeanne | Bowser | Ganondorf/Ganon | Quicksilver | Sweet Tooth | The Joker | Mewtwo | The Meta | Scout | Doomsday | Bane | Venom | Android 18 | Balrog | Silver Samurai | Shredder

DBX Combatants
Trish | Jeanne | Jango Fett | King Dedede | Sasuke Uchiha | Hiei | Liu Kang | Alucard | Predator | Meta Knight | Broly | Doomsday | Akuma | Magneto | Darth Vader | Vegeta | Zod | Demitri Maximoff |

Other
Wizard & Boomstick | Possessed Super Saiyan God Goku Pop Vinyl Figure | Sarge