The Dark Signers are the main antagonists of the Dark Signers arc of the TV show Yu-Gi-Oh! 5Ds, the second continuation of the Yu-Gi-Oh anime. Each one is a deceased individual revived by the Earthbound Immortals, enemies of the Crimson Dragon and the Signer Dragons. In their new states, the Dark Signers were given great power and a thirst for revenge. Each Dark Signer, with the exception of Roman and Rex Goodwin, had some sort of personal grudge against or connection to one of the five Signers. The Dark Signers' transformations derived from the Netherworld itself. Because of this, there was no way to undo the transformation and it was said the Dark Signers were no longer among the living. When a Dark Signer is defeated in a Shadow Game, instead of simply dying like they once did, their bodies instead turn to dust.

The Dark Signers were capable of using a distinctive type of monster called a Dark Synchro Monster, which were black-lined. Their signature cards are the Earthbound Immortals, which need the help of Field Spell cards to stay on the field. Each Dark Signer wields a different Earthbound Immortal and each Earthbound Immortal has different abilities.