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- Yagyuu Kyuubei: They first in Episode 275, Kyuubei was to a male. Later in Episode 277 she put a tracking chip on Kyuubei's sword early go in Episode 275 later in another planet turning everyone switch genders it turn out is Gintoki, Shinpachi, Kagura, Sadaharu, Kondo, Hijikata, Sougo, Sarutobi, Tsukuyo and Yagyuu Kyuubei they gender back to normal and fightback Kyuubei destroy their genderbender ray machine.
Her plan turning everyone in edo switch genders. Turning male in to female and female in to male. Later in Episode 277 she Kyuubei's sword with a tracking chip. One step ahead at their next location, purposely to get into the firing range of the cult's weapon and changes everyone back to normal, killing all the cult's members and destroy their weapon before any planet falls victim to their curse.
- Daishikyou has the same voice actress is Benio Amakusa from Ouran High School Host Club, Takuto Shindou and Rion Hyuuga from Inazuma Eleven Go and KT fromIxion Saga DT.