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These villains appear in the My Little Pony series. The original show (despite being infamous for its girly nature) had some rather dark and menacing villains, such as Tirek, Lavan, and Grogar (and Tirek and Lavan were killed, thus making My Little Pony's first generation one of the very few TV cartoons in the 1980s to kill characters off).
This stayed the same for the most part in the newer "Friendship is Magic" canon, with villains like Nightmare Moon, Sunset Shimmer, Discord, the Changelings and King Sombra.