Villains who are considerably less dark and evil than what is considered normal for the work in which they appear, having a comedic presence within a story that is otherwise serious; this is the exact opposite of, and mutually exclusive from, Game Changer. These villains are usually seen in TV shows, as they sometimes become more nasty, depending on the episode. In particular, villains who fall under the Mischievous, Incompetent, Dimwits or Ensemble categories are likely to be part of this category.
It is almost impossible for a Big Bad to be a Comic Relief, because if the main antagonist is comical, then by default most of the story's other elements will be comical as well and this trope cannot apply to the work. The only exception would be if a story primarily driven by realistic drama inexplicably had a comical or cartoonish bad guy who was the only recurrent antagonist but who was irrelevant to the rest of the plot (e.g. Santiago).
Comic reliefs can and tend to have their more threatening moments (such as when their true intentions are revealed, i.e. GLaDOS) but their comedy must be more prominent and/or remembered.
Pure Evils can never qualify because their acts are always serious and fearsome. This is applicable even if they appear to be funny because their humor is usually sadistic and makes them even more threatening (e.g. Hidan and Kefka Palazzo), while a Comic Relief's humor is genuinely played off to make them less threatening.
Comedy villains can never qualify unless there is a game changer in the story the villain is in, considering if a villain is in comic relief, it means the story is very serious but this villain lightens up the story, while comedy villains are in a story that is very comedy intended.