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They are anacondas from Borneo who consumed preys that consumed "blood orchid", a flower that extends lifespans. Therefore, they kept growing for their entire life and became gigantic. The females are much larger than the males.
Aside expanded lifespan, they are much faster, stronger and more agile than both normal anacondas and Amazon giant anacondas. They also have a great intelligence for animals and a highly aggressive behavior. Like the rest of anaconda in film series, they possessed super strengths and slighty different physiology as aside enhanced strength and agility, they able to devouring prey less than 5 second and able to digest large preys around few hours.
At the beginning of the film, a Borneo Anaconda attacks a native hunter in the jungle. He manages to flee, but he then falls into a river below and is dragged underwater by another Anaconda.
Some times later, dozens of male Anacondas come out of their territories during a mating season to find a female in her pit. However, a team of pharmaceutical researchers consisting of Dr. Jack Byron, Gordon Mitchell, Sam Rogers, Gail Stern, Cole Burris, Ben Douglas, and their guides Bill Johnson and Tran Wu, comes simultaneously in the area to search the "blood orchid". During a night, a male anaconda encounters Bill's pet monkey Kong and pursues him, but the monkey manages to escape. The following morning, one of the anacondas finds a crocodile's carcass and eat it. The next day, a snake attacks the team and kills Ben, but the others flee away. Later, another anaconda also attacks Bill's friend John Livingston on his boat and devours him.
The remaining team members arrive to a nearby deserted village, but there an anaconda devours alive Gordon, who has been paralyzed by Jack with a poisonous spider shortly before. They then go into the jungle and are attacked by another anaconda, who manages to eat Tran underwater before being killed by Sam. A second one appears soon after and captures Cole, but Bill successfully kills the snake with his knife.
The team later finds the blood orchids above the pit where the male anacondas are mating with the female. Jack obliges Sam to retrieve the orchids, but after she did it the backpack containing the orchids falls into the pit and Jack is paralysed by his spider while trying to seize it, causing him to fall into the pit and be devoured by the snakes. However, the female anaconda then attacks the other team members, but Gail tricks her into biting a fuel container and Cole shoots it with a flare, causing a chain reaction that kills all the anacondas.
- Lopak Hunter - Eaten by Borneo Anaconda
- Lead Lopak Hunter - Eatan by Borneo Anaconda
- Borneo Crocodile - Eaten by Borneo Anaconda
- Dr Ben Douglas - Eaten by Borneo Anaconda
- John Livingston - Eaten by Borneo Anaconda
- Gordon Mitchell - Eaten by Borneo Anaconda
- Tran Wu - Eaten by Borneo Anaconda
- Dr Jack Byron - Eaten by Borneo Anaconda
- In real life, Anacondas are not present in Borneo as they actually native of South America. Instead, some python species are the true native constrictor snake in Borneo.