They were regular guards who served Yzma and formerly worked from Emperor Kuzco (who was accidentally turned into a llama by Yzma). After getting rid of her former henchman Kronk, Yzma calls in her guards and orders them to kill Pacha and Kuzco (who turned into a llama) that they "murdered" the emperor. Kuzco tried to tell them that he was the emperor, but they didn't listen. Pacha took all the vials and dumped larger ones onto the guards, turning them into various animals such as a cow, a lizard, an octopus, a gorilla, an ostrich, and a warthog. One of the guards told Yzma that he wanted to go home because he turned into a cow. Yzma lets him leave, and asks if anyone else wants to go. The rest of the guards declined to leave, stating that they're good before chasing after Kuzco and Pacha.
Pacha first used a vial on Kuzco to turn him back to human, but Kuzco turned into a turtle instead. Pacha used the second vial, hoping Kuzco to be something with wings to avoid the guards at the bottom of the staircase. Kuzco turned into a bird and carried Pacha away, for a brief moment since he turned into a tiny red bird. He and Pacha crash into a wall and fell at the doorway. Kuzco picked the third vial that turned him into a whale, which caused the bridge they were standing on to break and made them fall into the palace moat. Yzma ordered the guards to drain the canals. The fourth vial turned Kuzco back to a llama before the canals were drained out of the palace's nose. Pacha and Kuzco held on to the side of the nose and Yzma told the guards to jump in after them. The guards jumped, but they slipped and lost their weapons and fell through the hole into the palace stairs. It is unknown what happened to them afterwards.